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. 2006 Dec 20;35(3):755–770. doi: 10.1093/nar/gkl1088

Figure 4.

Figure 4

Colony forming ability of yeast plasmids with or without geminiviral replicon at temperatures semi-restrictive for expression of RPA. [(a) (i)] and [(b) (i)] represent the sector diagram of the combinations of the yeast strains (W303a or ts mutants of RPA32 and 70) and transforming yeast plasmids (YCp50 or YCpO−-2A). The wild-type W303a yeast strain as well as ts mutants of RPA32 and RPA70 were transformed with either YCp50 vector (control) or with YCpO−-2A construct. The transformants were streaked on Ura− plates and allowed to grow at 33°C (permissive) (a, ii) and 35°C (semi-restrictive) (a, iii) temperatures in the case of RPA32 ts mutant. Similarly, the transformants were grown in Ura− plates at 30°C (b, ii) and 33°C (b, iii) temperatures for RPA70 ts mutant strains. Different colonies of ts mutant RPA32/A2F construct have been marked by # n. (c) and (d) Shows the percent stability of the plasmid YCp50 (control) and YCpO−-2A in the ts mutants of yeast for RPA32 and RPA70 respectively at different semi-restrictive temperatures. Inline graphic – represent YCp50 and Inline graphic represent YCpO−-2A.