Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Dec 20;35(3):755–770. doi: 10.1093/nar/gkl1088

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2006 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Yeast two-hybrid interaction of pea RPA32 and RPA70 with MYMIV-Rep_1–362. PsRPA32/RPA70 and wild-type Rep_1–362 as well as its mutant Rep_1–362 M2 (K227E substitution) were cloned in frame with activation domain (AD) and DNA-binding domain (BD) vectors and the combination of plasmids shown in panel (a) were co-transformed in yeast reporter strain AH109. The transformants were selected on synthetic media lacking leucine and tryptophan. (b) and (c) Show the growth of co-transformants on synthetic media lacking leucine/tryptophan and leucine/tryptophan/histidine respectively. β-Galactosidase assay of replica colonies of (c) on theYPD plate are shown in (d).