Table 1.

Primers used for isolation of RPA32 and RPA70 genes

Orientationa	Sequence of primer (5′→3′)b	Usage
S	TTY RYK VTT GAY GAY GGH ACM GG	Partial RPA32
A	CTC RTC SAW WGT YGA GTA TAT KHR MCC
S	CCG GAG CTC GAG CGC GCT AGC	5′ RPA32
A	CACAG GCC TGA CAG ACA AGG C
S	GGA ATA CAT GTT GAA GAA CTA GC	3′ RPA32
A	GTC GTC GAC ATA TGA TCA AGC TT
S	ATG GAT CCA TGT TCT CCA GCT CCC AAT TTG AC	RPA32 full-length
A	TTG AGT CGA CTC AAG CTT GTT TGT AGT GGG AGT C
S	TCA ARR GGA ARY TTG ARR CCT GC	Partial RPA70
A	CCR WTC ATS WWA GGR CAA GC
S	CTT ACG AGC CTG GTT TGA TCA AG	5′ RPA70
A	GTC ACA GGC CTG ACA GAC AAG GC
S	GGA ATA CAT GTT GAA GAA CTA GC	3′ RPA70
A	GAA GGA TTC ACA GAT GTC ACA ACC
S	CAT GGA TCC ATG TCG GTG AAT CTC ACG GCG AG	RPA70 full-length
A	TTG AGT CGA CTT ACT TCC TAC CGA ACT TGG AAA TC

^aS, sense; A, antisense.

^bThe primers underlined contained BamHI restriction site in the forward primer and SalI in the reverse primer preceded by a stop codon.