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. 2007 Jan 23;35(3):923–929. doi: 10.1093/nar/gkl1130

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© 2007 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — U6 snRNA and Lsm8p are delocalized in rsl nuclear import mutant cells. (A–C) U6 localization was investigated in rsl1-1ts (PSY871) cells at (A) 30°C or (B) shifted to the restrictive temperature (37°C) for 2 h or (C) in wild-type (WT) cells grown at 37°C. (D) rsl1-1ts cells were grown at permissive temperature (30°C) or (E) shifted to the restrictive temperature (37°C) for 2 h and U3 localization was tested to control for nuclear integrity. (F–I) GFP-Lsm8p localization was investigated in live rsl1-3ts (PSY1102) or wild-type (PSY580) cells carrying pMPS8 (encodes GFP-Lsm8) at (F,H) 30°C or (G,I) shifted to the 37°C for 1 h. (J–M) GFP-Lhp1p localization was investigated in live rsl1-3ts (PSY1102) or wild-type (PSY580) cells carrying pMPS2 (encodes GFP-Lhp1) grown at (J,L) 30°C or (K, M) shifted to the 37°C for 2 h. In the case of GFP-Lhp1p, a bright field view is presented to show the outline of the cells. Scale bar, 10 μm.