Figure 2.

Effect of spermidine and protein combinations on HJ resolution directionality and efficiency in excisive recombination. (A) Directionality is defined in context to the pathway from which the HJ substrates were obtained. For excision, attL and attR are (original) substrates while attP and attB are products. A ratio of 1 (dotted line) represents an equal amount of ‘substrates’ (attL/attR) and products (attP/attB) and indicates no bias; a ratio of >1 represents more products than substrates, indicating ‘forward’ resolution bias, while a ratio of <1 represents fewer products than substrates, indicating ‘reverse’ resolution bias. % Res refers to % resolution, and indicates the extent of HJ converted to substrates and products in the presence of the specified proteins. (B) Example of the actual data used in (A). The attL substrate used to make the Holliday junctions was double end-labeled, while the attR substrate was unlabeled; thus, only 3 of the 4 resolution products are visible. The ratio of products to substrates is determined as the (% attP+attB counts/% attL counts). The * represent uncharacterized side products.