Figure 6.

Effect of overlap sequence, polycations and proteins on excision HJ resolution. (A) Effect on HJ resolution by Int alone. Bars indicate bias of directionality while the numbers below indicate overall % resolution obtained (% Res), regardless of the direction of resolution. The sequence of the overlap region is given below the name of the junction. (B) Effect on HJ resolution by Int together with IHF and Xis. Data is formatted exactly as in part A. All reactions were performed as described in Materials and Methods and incubated at 37°C. The average of three independent experiments is shown. The dotted line denotes a product:substrate ratio of 1 (no resolution bias).