Figure 5.

Hybrids between RliE and mRNA targets. (A) For each hybrid, the upper part shows the region of RliE (red) that pairs with the mRNA (black). Numbers in parenthesis indicate the first and the last bases of RliE involved in the pairing. The lower part shows the predicted pairings of RliE with the mRNA at the nucleotide level. Boxes indicate translation start codons, and S.D. the Shine–Dalgarno sequence is shown. (B) In vitro duplex formation between RliE and predicted mRNA targets indicated at the top of each panel. At the top of each lane, numbers indicate the concentration used (nM) of unlabeled RNA with <1 nM of labeled RliE. Unbound radioactive RliE* is indicated.