Figure 6.

Hybrids between RliI and mRNA targets. (A) For each hybrid, the upper part shows the region of RliI (red) that pairs with the mRNA (black). On the nucleotidic sequence, the translation start codon of lmo2659 is indicated by a grey box, the translation stop codon of lmo2660 by an open box. Data are otherwise presented as in Figure 5A. (B) Duplex formation between RliI and lmo1035 mRNA. Data are presented as in Figure 5B. (C) northern blots on 15 µg of total RNA extracted from L. monocytogenes EGD-e (WT), EGD-e harboring the empty vector pAT18 (vector) and pAT18 carrying the rliI gene (RliI) grown to exponential (E) or stationary (S) phase in BHI at 37°C. The RNA probed is indicated on the left of each panel. RliI is probed with a specific oligonucleotide, lmo1035 and lmo1036 transcripts were probed with PCR fragments. Estimated sizes of the transcripts are indicated on the right side of each panel. As loading control, gels were stained with EtBr before transfer to evaluate 16S rRNA levels (16S RNA).