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. 2007 Jan 10;35(3):850–860. doi: 10.1093/nar/gkl735

Figure 3.

Figure 3

Protein crosslinking by DIDS. (A) N60-ttMutS2. (B) ttMutS2. (C) ttSmr. (D) BSA. N60-ttMutS2 (2 μM), ttMutS2 (2 μM), ttSmr (10 μM) and BSA (2 μM) were incubated with homo-bifunctional crosslinker DIDS at 37°C for 30 min. The concentration of DIDS is indicated at the top of figure. Reaction mixtures were subjected to 7.5 or 12% SDS–PAGE and stained with Coomassie blue. Arrows indicate crosslinked dimeric proteins. The calculated molecular mass of dimeric N60-ttMutS2, ttMutS2 and ttSmr were 120, 165 and 30 kDa, respectively.