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. 2007 Jan 10;35(3):850–860. doi: 10.1093/nar/gkl735

Figure 8.

Figure 8

The effect of substitution of His-701 with an alanine residue. (A) pT7Blue plasmid DNA was incubated with the indicated concentration of wild-type ttMutS2 (WT ttMutS2) or H701A at 37°C for 20 min. (B) 32P-labeled 20 bp dsDNA was incubated with the indicated concentration of ttMutS2 at 37°C for 120 min. Initial rates (vo) were calculated based on the proportion of all products to unreacted substrates and then plotted against the protein concentration. Triangles and circles are wild-type ttMutS2 and H701A, respectively.