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MKP-1 nascent transcripts after induction by TRH. Nascent transcripts of MKP-1 were purified from chromatin prepared from GH4C1 cells collected at various times after stimulation of 100 nM TRH. Transcripts which include MKP-1 exon 1, exon 2 and exon 4 sequences were quantified by real-time RT-PCR. A typical experiment repeated twice (mean ± SD in triplicates) is shown.
