Figure 3.

DRB inhibits recruitment of P-TEFb and dynamic redistribution of DSIF on the MKP-1 gene. Densities of NELF, DSIF and P-TEFb on the MKP-1 gene were assessed at various time points of TRH stimulation in the absence (filled bars) or presence (hatched bars) of DRB. ChIP assay was performed with an anti-NELF-A (A), an anti-Spt5 (B) and an anti-cyclin T1 antibody (C). Density on the MKP-1 gene was analyzed by real-time PCR and presented as a percentage of input. A typical experiment (mean ± SD, n = 3) repeated three times is shown.