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. 2007 Jan 26;35(3):1007–1017. doi: 10.1093/nar/gkl1138

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Phosphorylation by P-TEFb of Serine-2 in the CTD pol II is essential for transcriptional elongation. The density of total and CTD-phosphorylated pol II on the MKP-1 gene was assessed at various time points of TRH stimulation in the absence (filled bars) or presence (hatched bars) of the DRB. ChIP assay was performed with specific anti-pol II antibodies: (A) an anti-pol II antibody (N-20), which recognizes N-terminal region of pol II; (B) an anti-pol II antibody (H14), directed against phospho-Ser-5 CTD; and (C) an anti-pol II antibody (H5), directed against phospho-Ser-2 CTD. Density on the MKP-1 gene was quantified by real-time PCR and is presented as a percentage of input. A typical experiment (mean ± SD, n = 3) repeated three times is shown.