Figure 4.
D2 receptor-induced phosphorylation of MAPK and CREB in wild-type and DARPP-32-deficient mice. (A) Immunoblots of phospho-MAPK in brain slices from wild-type and DARPP-32-deficient mice. Brain slices were incubated with or without quinpirole (60 μM) for 15 min. Extracts of slices were immunoblotted with anti-phospho-MAPK antibody. (B) Quantitation of quinpirole-induced phosphorylation of p42 MAPK. ∗, P < 0.01, compared with minus quinpirole. (C) Immunoblots of phospho-CREB in brain slices from wild-type and DARPP-32-deficient mice. Brain slices were incubated with or without quinpirole (60 μM) for 15 min. Extracts of slices were immunoblotted with anti-phospho-CREB antibody. (D) Quantitation of quinpirole-induced phosphorylation of CREB. ∗, P < 0.01, compared with minus quinpirole.