Figure 2.

Elimination and appearance of BrdUrd-labeled SVZ cells after Ara-C treatment. (A) Number of BrdUrd-positive nuclei counted in whole mounts of the lateral wall of the lateral ventricle at different survivals after pump removal in infused (i, solid line) and contralateral (ni, dashed line) hemispheres and in untreated (dotted line) mice. Error bars show the SE. (B) Distribution of BrdUrd-positive nuclei in whole-mount preparation of injected hemisphere 0.5 d after pump removal. BrdUrd-positive cells are more prevalent in the anterior horn where the SVZ is thicker, but reappear throughout the SVZ. BrdUrd-positive nuclei initially appear away from the infusion site (white area in the dorsal SVZ), suggesting that more cells were killed in that region or that some Ara-C remains in the tissue. (Scale bar = 500 μm.)