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. Author manuscript; available in PMC: 2007 Mar 2.
Published in final edited form as: Biochemistry. 2006 Oct 3;45(39):11727–11736. doi: 10.1021/bi061180d

Figure 3.

Figure 3

Calcium and substrate dependence of Cl-amidine-induced inactivation. (A) PAD4 was preincubated with increasing concentrations of Cl-amidine in the absence and presence of calcium and then BAEE added to initiate the IC50 assay. Calcium was also added at this time to the sample preincubated in the absence of this metal ion to upregulate the activity of PAD4. (B) Substrate protection was assayed by observing the time-dependent inactivation properties of Cl-amidine (100 μM). For these studies, product formation in the presence and absence of Cl-amidine was quantified as a function of time using two different concentrations of BAEE (2 and 10 mM) as the substrate.