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. Author manuscript; available in PMC: 2007 Mar 2.
Published in final edited form as: Methods Enzymol. 2005;394:175–209. doi: 10.1016/S0076-6879(05)94007-X

Figure 2.

Figure 2

IPAP-HSQC pulse sequence (Ottiger, 1998). The phase cycle is: ϕ1 = −y,y; ϕ2 = x,x,−x,−x (in–phase spectrum) and ϕ2 = −y,−y,y,y (anti–phase spectrum); ϕ3 = 4(x),4(y),4(−x),4(−y); ϕ4 = 8(x),8(−x); reciever = x,−x,−x,x (in–phase spectrum) and x,−x,−x,x,−x,x,x,−x (anti–phase spectrum). To achieve quadrature detection in t1, States-TPPI incrementation is applied to ϕ2 and ϕ3. The portion of the pulse sequence between the arrows (↑) is ommitted for the in–phase spectrum. The final pulse on 1H during the INEPT transfer is a 3919 compound pulse, used for water suppression. Gradients employed are: G1 = (4 G/cm, 1 ms) ; G2 = (26 G/cm, 0.25 ms) ; G3 = (24 G/cm, 1 ms).