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. Author manuscript; available in PMC: 2007 Mar 2.
Published in final edited form as: Methods Enzymol. 2005;394:175–209. doi: 10.1016/S0076-6879(05)94007-X

Figure 3.

Figure 3

The HNCA-ECOSY pulse sequence. The phase cycle is: ϕ3=x,−x; ϕ2=x,x,−x,-x; ϕ3=x,x,x,x,x,x,x,x,−x,−x,−x,−x,−x,−x,−x,−x; ϕ4=x; ϕ5=x,x,x,x,−x,−x,−x,−x; ϕ6=x; reciever = x,−x,−x,x,x,−x,−x,x,−x,x,x,−x,−x,x,x,−x. Quadrature detection in t2 is achieved by cycling ϕ1 in States-TPPI fashion. Quadrature detection in t1 is achieved by States-TPPI cycling of ϕ4 and ϕ5. Gradients employed are: G1 = (20 G/cm, 0.5 ms) ; G2 = (20 G/cm, 1 ms) ; G3 = (12 G/cm, 1 ms) ; G4 = (26 G/cm, 1 ms) ; G5 = (26 G/cm, 1 ms) ; G6 = (28 G/cm, 1 ms) ;