IPAP-HNCO of PF0385 under isotropic (Panel A) and aligned (Panel B) conditions. The samples were 1 mM PF0385 in 20 mM Sodium phosphate, 15 mM KCl, 90%H2O for isotropic and the same buffer, 85 mM NaCl, 10 mg/mL Pf1 phage for aligned. The peaks corresponding to the sum and difference spectra are colored red and black, respectively.