Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2007 Mar 2.

Published in final edited form as: Mol Cell Endocrinol. 2006 Dec 8;264(1-2):128–141. doi: 10.1016/j.mce.2006.11.002

Effect of ERRα and ERRγ on the AAB activity. A. Dose effect. The AAB-Luc reporters were cotransfected with myc-ERRα or myc-ERRγ individually or together (1:1 ratio) at the varying concentration (as indicated) into HEC1-B cells. Twenty-four h after transfection, cells were collected and reporter Luc activities measured. The firefly luciferase reporter activities were normalized to Renilla luciferase activities and plotted as fold of activation against vector control. The results are reported as the mean ± S.D. from a minimum of three independent experiments with duplicates for each experiment. Expression level of the transiently transfected ERRα and ERRγ expression constructs were verified with myc antibody by Western blotting (insert). B. Effect of ERRα on ERRγ transactivation function with AAB. Mutant ERRα (either truncation or mutation) were cotransfected with ERRγ expression vector (or empty vector) and the AAB-Luc reporters into HEC-1B cells. The Luc activities were determined 24 h later. Schematic presentation of the ERRα constructs is shown at the bottom. 1–422, full-length ERRα; 1–173, N-terminal region of the ERRα (1–173 amino acid); 76–422, C-terminal region of the ERRα (76–422 amino acid); P-boxmut, mutation made at the P-box of the DBD. Data is presented as means ± SE from three experiments with duplicated samples.

Effect of ERRα and ERRγ on the AAB activity. A. Dose effect. The AAB-Luc reporters were cotransfected with myc-ERRα or myc-ERRγ individually or together (1:1 ratio) at the varying concentration (as indicated) into HEC1-B cells. Twenty-four h after transfection, cells were collected and reporter Luc activities measured. The firefly luciferase reporter activities were normalized to Renilla luciferase activities and plotted as fold of activation against vector control. The results are reported as the mean ± S.D. from a minimum of three independent experiments with duplicates for each experiment. Expression level of the transiently transfected ERRα and ERRγ expression constructs were verified with myc antibody by Western blotting (insert). B. Effect of ERRα on ERRγ transactivation function with AAB. Mutant ERRα (either truncation or mutation) were cotransfected with ERRγ expression vector (or empty vector) and the AAB-Luc reporters into HEC-1B cells. The Luc activities were determined 24 h later. Schematic presentation of the ERRα constructs is shown at the bottom. 1–422, full-length ERRα; 1–173, N-terminal region of the ERRα (1–173 amino acid); 76–422, C-terminal region of the ERRα (76–422 amino acid); P-boxmut, mutation made at the P-box of the DBD. Data is presented as means ± SE from three experiments with duplicated samples.