Table I.

Plasmids

Reporters	Description	Reference
AAB-TATA-Luc	hERRα MHRE	(Liu et al., 2003)
3x ERE-TATA-Luc	synthetic triple ERE	(Zhang & Teng, 2000)
1x ERE-TATA-Luc	vit A2 ERE	(Zhang & Teng, 2000)
NRRE-TATA-Luc	MCAD, NRRE	(Disch et al., 1996)
Expression constructs
Plasmid	Description	Reference
pcDNA3.1-ERRα-myc-his	with myc and his tag	(Zhang and Teng, 2000)
pSG-ERRα	hERRα	(Yang et al., 1998)
pFlag-ERRα	with flag tag	(Ichida et al., 2002)
pGEX-4T-1	GST tag expression vector	Amersham
GST-ERRα	GST tag	(Yang et al., 1996)
pcDNA3.1-ERRγ	hERRγ	(Hentschke et al., 2002)
pcDNA3.1-myc-ERRγ	myc tag	(Hentschke et al., 2002)
GST-ERRγ	GST tag	(Hentschke et al., 2002)
GST-ERRγ ΔAF2	delete AF2	(Hentschke et al., 2002)
pSG-HEGO		(Migliaccio et al., 1991)
pcDNA3.1-ERβ		(Hall and McDonnell, 1999)
RORα		(Giguere et al., 1994)
Mutant constructs
Construct	Mutation	Reference
ERRα-N	N-terminal 1–174	(Kraus et al., 2002)
ERRα-C	C-terminal 76–422	(Kraus et al., 2002)
ERRα - pboxmut	E97G/A98S/A101V	(Kraus et al., 2002)
GST-ERRα ΔAF2	hERRα (1–406, delete 16aa)	current study
Coactivator expression constructs
Construct	Description	Reference
PCR3.1-hSRC-1a	human	(Onate et al., 1995)
pcDNA3.1-hPGC-1α	human	(Knutti et al., 2000)