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. Author manuscript; available in PMC: 2007 Mar 2.
Published in final edited form as: Mol Cell Endocrinol. 2006 Dec 8;264(1-2):128–141. doi: 10.1016/j.mce.2006.11.002

Table II.

Primers

For Northern blotting
hERRα
Direction Exon Sequence
forward exon 1 5′-ACA AGC AGC CGG CGG CGC CGC CGA GTG A3′
reverse exon 2 5′AT GTC GAC TCC TCC TCT TCC TTG3′
forward exon 6 5′AGA TGT CAG TAC TGC AGA GCG T3′
reverse Exon 7 5′GCT TCA TAC TCC AGC AGG3′
hERRγ
forward exon 1 5′ATG TCA AAC AAA GAT CGA CAC3′
reverse exon 2 5′ GCC CAC TAC CTC CCA GGA TA3′
hPGC-1α
forward exon 2 5′TTT GCC CAG ATC TTC CTG AAC3′
reverse exon 3 5′ATG AGG CCA ATC CGT CTT C3′
hβ-Actin
forward exon 5 5′GAC AGG ATG CAG AAG GAG ATC AC3′
reverse exon 6 5′GCT GAT CCA CAT CTG CTG GAA3′
For real time PCR
mERRα
forward exon 6 5′TTC GGC GAC TGC AAG CTC3′
reverse exon 7 5′CAC AGC CTC AGC ATC TTC AAT G3′
mβ-Actin
forward exon 5 5′GAC AGG ATG CAG AAG GAG ATT AC3′
reverse exon 6 5′GCT GAT CCA CAT CTG CTG GAA3′
mERRγ
Applied Biosystems assay ID Mm00516267_m1
mPGC-1α
Applied Biosystems assay ID Mm00447183_m1
For EMSA
1x ERE
top strand 5′CCC GAA GCT TCT AGG TCA CAG TGA C3′
bottom strand 5′GC TCG AGG TCA CTG TGA CCT AGA AG3′
For ChIP assay
MHRE region
forward 5′GTC AGT GCA GGA CAG CCC GCG AG3′
reverse 5′GAT AGG GCC CGG ACG GAG AAA GC3′
4 kb upstream
forward 5′GCA TAG CGA CAC TCG GGA CCT3′
reverse 5′GCG GGT CTT GCT AAT GTT G3′