For Northern blotting |
hERRα |
Direction |
Exon |
Sequence |
forward |
exon 1 |
5′-ACA AGC AGC CGG CGG CGC CGC CGA GTG A3′ |
reverse |
exon 2 |
5′AT GTC GAC TCC TCC TCT TCC TTG3′ |
forward |
exon 6 |
5′AGA TGT CAG TAC TGC AGA GCG T3′ |
reverse |
Exon 7 |
5′GCT TCA TAC TCC AGC AGG3′ |
hERRγ |
forward |
exon 1 |
5′ATG TCA AAC AAA GAT CGA CAC3′ |
reverse |
exon 2 |
5′ GCC CAC TAC CTC CCA GGA TA3′ |
hPGC-1α |
forward |
exon 2 |
5′TTT GCC CAG ATC TTC CTG AAC3′ |
reverse |
exon 3 |
5′ATG AGG CCA ATC CGT CTT C3′ |
hβ-Actin |
forward |
exon 5 |
5′GAC AGG ATG CAG AAG GAG ATC AC3′ |
reverse |
exon 6 |
5′GCT GAT CCA CAT CTG CTG GAA3′ |
For real time PCR |
mERRα |
forward |
exon 6 |
5′TTC GGC GAC TGC AAG CTC3′ |
reverse |
exon 7 |
5′CAC AGC CTC AGC ATC TTC AAT G3′ |
mβ-Actin |
forward |
exon 5 |
5′GAC AGG ATG CAG AAG GAG ATT AC3′ |
reverse |
exon 6 |
5′GCT GAT CCA CAT CTG CTG GAA3′ |
mERRγ |
Applied Biosystems assay ID Mm00516267_m1 |
mPGC-1α |
Applied Biosystems assay ID Mm00447183_m1 |
For EMSA |
1x ERE |
top strand |
5′CCC GAA GCT TCT AGG TCA CAG TGA C3′ |
bottom strand |
5′GC TCG AGG TCA CTG TGA CCT AGA AG3′ |
For ChIP assay |
MHRE region |
forward |
5′GTC AGT GCA GGA CAG CCC GCG AG3′ |
reverse |
5′GAT AGG GCC CGG ACG GAG AAA GC3′ |
4 kb upstream |
forward |
5′GCA TAG CGA CAC TCG GGA CCT3′ |
reverse |
5′GCG GGT CTT GCT AAT GTT G3′ |