Figure 2. Protein expression of cardiac L-VDCC subunits in old wild-type and tg Ca_V1.2 mice.

(a) Specimens from old wild-type mice and tg Ca_V1.2 in heart failure were analyzed in immunoblots using specific polyclonal antibodies directed against the particular L-VDCC subunits.

(b) Protein expression of L-VDCC subunits was always normalized to cardiac calsequestrin protein expression in the same sample. Quantitative analysis of subunit protein expression is depicted as ratio of 10 months old tg Ca_V1.2 vs. age-matched wild-type. β₁ protein bands were faint, and thus not analyzed quantitatively (number of WT/old tg Ca_V1.2 specimens was always identical for each subunit; n = 4). * p<0.05.