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. Author manuscript; available in PMC: 2008 Jan 23.
Published in final edited form as: FEBS Lett. 2007 Jan 2;581(2):309–314. doi: 10.1016/j.febslet.2006.12.033

Figure 1.

Figure 1

Recognition of Asp-tRNAAsn and Asp-tRNAAsp by GatCAB. (A) Time course of transamidation of Asp-tRNAAsn (o) and Asp-tRNAAsp (●). [14C]Asp-tRNAAsn or [14C]Asp-tRNAAsp (0.5 μM) were incubated with M. thermautotrophicus GatCAB (10 nM) at 37°C. (B) RNase A protection analysis of GatCAB:Asp-tRNA complexes. [3H]Asp-tRNAAsn (o) (10 nM) and [3H]Asp-tRNAAsp (●) (10 nM) were mixed with 0-2000 nM of GatCAB (A) and treated with RNase mix (10μg) for 30 seconds at 4°C. The residual radioactivity on the filter after TCA washing was determined as described in Materials and Methods.