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. Author manuscript; available in PMC: 2008 Jan 23.
Published in final edited form as: FEBS Lett. 2007 Jan 2;581(2):309–314. doi: 10.1016/j.febslet.2006.12.033

Figure 2.

Figure 2

Effect of domain swap tRNAAsn mutants on transamidation activity and binding to GatCAB. (A) Clover-leaf representation of M. thermautotrophicus tRNAAsn and tRNAAsp. Sequence differences between the two tRNAs are circled. (B) Relative transamidation activities of various Asp-tRNAAsx mutants and wild-type Asp-tRNAAsn. Transamidation activity was measured as described in Materials and Methods with 1 μM wild-type Asp-tRNAAsn or mutant Asp-tRNAAsx and relative activity was expressed as ratio of conversion rate of wild-type Asp-tRNAAsn to Asn-tRNAAsn. The T-stem* swap did not include the first base pair of the stem. (C) RNase A protection analysis of GatCAB:Asp-tRNA complexes. Wild type Asp-tRNAAsn (o) and swap tRNAAsn mutant with T-arm (●) or anticodon arm (▲) portion of tRNAAsp. The curves were fitted from the experimental points.