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. Author manuscript; available in PMC: 2008 Jan 23.
Published in final edited form as: FEBS Lett. 2007 Jan 2;581(2):309–314. doi: 10.1016/j.febslet.2006.12.033

Figure 3.

Figure 3

Transamidation by H. pylori or M. thermautotrophicus GatCAB with homologous and heterologous Asp-tRNAAsn. (A) Clover-leaf presentation of H. pylori tRNAAsn and tRNAAsp species. The important identity elements [16] are boxed. The two additional U residues in the D-loop are shown in bold. (B) Transamidation of M. thermautotrophicus and H. pylori Asp-tRNAAsn by a bacterial (H. pylori) or archaeal (M. thermautotrophicus) GatCAB. Reactions were carried out over 5 minutes with 10 nM enzyme. Activities are plotted relative to the homologous reaction; e.g., the activity of the H. pylori GatCAB with the M. thermautotrophicus Asp-tRNAAsn was relative to the activity of the H. pylori with its own Asp-tRNAAsn.