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Diagram of the constructs used in this study. All Nef variants were under control of the CaMV 35S promoter. Sp: PR1 signal peptide; club: myristoylation site; tridents: predicted glycosylation sites; Δg: unglycosylated Nef forms. P27: nef full-length cDNA; p27 mut: mutated form of the p27 cDNA in which the myristoylation consensus sequence is abolished; p25: nef cDNA encoding truncated Nef.
