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Western blot analysis of the expression level and phosphorylation status of Jak-2 (A), and of the expression of SOCS-1 (B) in ESTDAB-004 cell line. We observed that Jak-2 was present but its phosphorylation was very low and was accompanied by a high SOCS-1 expression (negative regulator of IFN-γ response). Arrows indicate the specific proteins. Nuclear extracts as a positive control (C+) for Jak-2 were obtained from Cell Signaling.
