Figure 4.

LOH and frameshift analysis was performed on HNPCC tumors that simultaneously lost HLA class I and β2m expression. LOH markers: (see legend from Figure 3 for key). Frameshift markers:HLA A: 1 – 4^th exon 7(C) 2 – 5^th exon 3 (GGA); HLA – B: 3(GA) & 3(CA); β2m: 1 – 1^st exon 4(CT), 2 – 2^nd exon 4(GA). & 5(A), 3 – 2^nd exon 5(A) (key: ins – insertion; del – deletion; 0 – no mutation; IHC -immunohistochemistry)