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. 2007 Mar 2;3(3):e30. doi: 10.1371/journal.pcbi.0030030

Figure 1. Effective Modification of Ppara mRNA and Its Known Target Genes by siRNA In Vivo.

Figure 1

Ppara was monitored with three probes, Ppara 1, 2, and 3, targeting different fragments of the whole Ppara transcript. The mRNA levels of Ppara, as detected by all three Ppara probes (A), and by its known target genes Cpt1a (B) and Acadl (C) decreased coordinately in the livers of mice treated with Ppara siRNA1 and Ppara siRNA2. The mRNA levels of Ppara and its known target genes, Cpt1a or Acadl, were also measured in Ppara −/− mice as a positive control for the silencing effect of Ppara siRNA. Similar concordant regulation between Ppara and its known targets is observed in Ppara −/− mice when compared with wild-type mice at day 1 (D1) or day 7 (D7) after vehicle treatment. The mRNA level for each mouse is indicated by a vertical bar.