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. Author manuscript; available in PMC: 2008 Mar 2.
Published in final edited form as: J Mol Biol. 2006 Dec 12;366(4):1266–1281. doi: 10.1016/j.jmb.2006.12.015

Figure 3.

Figure 3

(a) The reference 1D 1H NMR spectrum of UDP-TEMPOH in deuterated buffer solution. The asterisk in the spectrum indicates acetone, which was used as a chemical shift reference. An exponential weighting function of 2 Hz was applied to mimic the linewidth of UDP-TEMPOH in the STD spectrum. (b) The STD spectrum of UDP- TEMPOH with GnT-V. (c) The normalized percentages calculated from the STD and reference spectra of UDP- TEMPOH are superimposed on the chemical structure of UDP- TEMPO. Peaks from H2’ and H3’ are overlapped giving an average STD value.