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NMR and sample conditions for STD experiments.
| Substrate | Acceptor | Acceptor with 10 mM UDP | UDP-GlcNAc | UDP-TEMPOH |
|---|---|---|---|---|
| Concentration (mM) | 2.0 | 2.0 | 2.5 | 1.5 |
| GnT-V concentration (μM) | 100 | 100 | 100 | 60 |
| Magnet strength (MHz) | 900 | 900 | 600 | 600 |
| Saturation bandwidth (Hz) | 100 | 100 | 100 | 50 |
| Saturation frequency (ppm) | 0.0 | 0.0 | 0.0 | 7.1 |
| Saturation timea (s) | 0.5 | 0.5 | 2.0 | 1.9 |
The total saturation time includes the delay (1 ms) between the Gaussian pulses in the cycled pulse train.
