Figure 3. mGluR Activation Increases APP Translation in SNs.

(A) Immunoprecipitated, ³⁵S-labeled APP (120-kDa band) from WT (15 min) and KO and WT (60 min) SNs analyzed by SDS-PAGE and (B) plotted as a percentage of APP synthesis; n = 3 repetitions. Asterisk indicates significant differences, with p = 0.008 between ±DHPG samples at 15 min and p = 0.016 between control at 15 min and DHPG at 60 min. For the control samples at 15 and 60 min, p = 0.056, and for the samples with or without DHPG at 60 min, p = 0.05. (C) Immunoprecipitated, ³⁵S-labeled APP (120-kDa band) from WT SNs treated with DHPG, anisomycin, and MPEP, analyzed by SDS-PAGE, and (D) plotted as a percentage of APP synthesis; n = 3 repetitions (DHPG), n = 4 (anisomycin + DHPG and anisomycin), and n = 5 (MPEP + DHPG and MPEP).