Table 1.
| PC±SD (P) | HNE±SD (P) | 3-NT±SD (P) | ||
|---|---|---|---|---|
| Control | 100±0.3 | 100±0.3 | 100±0.3 | |
| HNE | 129±1.4 (0.02) | 129±1.4 (0.05) | 129±4.1 (0.05) | |
| 75 μM | + 50 μM | 100±2.8 (0.05) | 101±3.4 (0.05) | 101±5.1 (0.05) |
| ALCAR | LA | |||
| HNE plus | HNE plus | |||
| ALCAR | LA | |||
| (μM) | (μM) | |||
| 50 | 50 | 126±1.9 (0.03) | 128±1.9 (0.05) | 125±5.4 (0.09) |
| 50 | 75 | 125±2.8 (0.05) | 125±3.4 (0.05) | 123±5.1 (0.10) |
| 50 | 100 | 123±3.9 (0.07) | 122±4.4 (0.10) | 120±7.2 (0.17) |
| 75 | 50 | 112±0.3 (0.005) | 111±0.7 (0.005) | 114±1.9 (0.005) |
| 75 | 75 | 111±1.1 (0.04) | 112±0.1 (0.005) | 114±2.6 (0.05) |
| 100 | 75 | 112±1.1 (0.04) | 112±0.1 (0.005) | 113±3.7 (0.12) |
| 100 | 100 | 113±3.5 (0.14) | 112±0.9 (0.05) | 114±4.1 (0.14) |
| 75 | – | 120±1.7 (0.02) | 121±1.4 (0.05) | 119±4.1 (0.05) |
| – | 50 | 121±1.2 (0.02) | 122±1.8 (0.05) | 121±2.1 (0.05) |
Protective effect of ALCAR+LA against HNE-induced oxidative stress. The treatment of cortical neuronal cultures is as described in “Materials and Methods” section. PC and P, refer to protein carbonyls and p value respectively. The data are the mean ± SD expressed as percentage of control values. Each experiment was repeated three times with five independent samples. Statistical comparison was by ANOVA (n=5). Post hoc analysis was via Student-Newman-Keuls test, and the P values given are compared with the control.