Table 1.
Variable region genes used in 15 anti-DNA antibodies and the combined R/S ratio for all CDRS except CDR3 of the heavy chain. The variable region genes marked in italics have previously been described in IgG anti-DNA antibodies [12] Variable region genes were assigned using VBASE [21] in the case of VH, VL, and JL. D region genes and JH genes were assigned by a combination of VBASE and manual comparison
| Antibody code | VH family | VH Locus | D Locus | JH Locus | VL Locus* | JL Locus | Total R/S ratio for the CDRS of the heavy and light chain combined |
|---|---|---|---|---|---|---|---|
| R5C08 | 1 | 1–46 | D3-22 | JH5B | A20 | Jκ4 | 2·0 |
| R4C17 | 3 | 3–11 | D-5–24 | JH4A | A27(2) | Jκ1 | 6·0 |
| R4C09 | 3 | 3–9 | D4-11/4/17 | JH5B | A27(1) | Jκ1 | 3·5 |
| R4C12 | 3 | 3–9 | D6-? | JH5B | A27(1) | Jκ1 | 2·0 |
| R5C02 | 1 | 1–46 | D3-22 | JH3B | A27(1) | Jκ1 | 1·4 |
| P253 | 1 | 1–8 | D1-1/D1-20 | JH5B | B3(2) | Jκ4 | 7·0 |
| R5C20 | 3 | 3–7 | A20 | Jκ4 | 2·7 | ||
| R4C07 | 1 | 1–46 | D3-22 | JH3B | A27(1) | Jκ1 | 1·5 |
| P212 | 3 | 3–33 | D3-3 | JH3B | B3(1) | Jκ4 | 4·3 |
| R4C15 | 3 | 3–9 | D7-27 | JH3B | A27(1) | Jκ1 | 2·3 |
| R4C03 | 3 | 3–48 | D3-16 | JH4B | A20 | Jκ4 | 2·4 |
| R5C06 | 3 | 3–9 | D4-23 | JH5B | A27(2) | Jκ1 | 4·3 |
| R4C04 | 3 | 3–33 | D3-3 | JH4A | A27(2) | Jκ1 | 4·0 |
| R5C16 | 3 | 3–30 | D7-27 | JH6a/b/c | A20 | Jκ4 | 3·8 |
| R5C09 | 1 | 1–69 | D4-17 | JH3A | A20 | Jκ4 | 4·2 |
| Mean | 3·4 |
*
Numbers in parenthesis indicate the light chain somatic variant used.