Fig. 1.

(a) Dose–response curve (n = 3). Purified peripheral blood eosinophils (0·4 × 10⁶/ml) from healthy subjects were incubated with rhIL-5 (0–1000 ng/ml) for 120 min at +37°C. Results are presented as change in the proportion of IL-5Rα positive eosinophils as compared to the RPMI control. (b) Dose–response curve (n = 3). Purified peripheral blood eosinophils (0·4 × 10⁶/ml) from healthy subjects were incubated with rhGM-CSF (0–2000 ng/ml) for 120 min at +37°C. Results are presented as change in the proportion of IL-5Rα positive eosinophils as compared to the RPMI control.