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Purified peripheral blood eosinophils (0·4 × 106/ml) from healthy subjects (n = 3) were incubated with rhIL-5 (10 ng/ml) (○,□,▵) or RPMI (•,▪,▴) for 0–120 min at +37°C. Results are presented as the proportion of IL-5Rα positive eosinophils.