Fig. 5.

RPMI or PF (500 µl) from the patient with PFE was preincubated with anti-IL-5 (5 µg/ml), anti-GM-CSF (5 µg/ml) or the IgG1 isotypic control (5 µg/ml), for 60 min at +37°C. Thereafter purified peripheral blood eosinophils (0·4 × 10⁶/ml) from healthy subjects (n = 3) were added. When both antibodies were used in PF (5 µg/ml each), the isotypic control antibody was used at 10 µg/ml. Eosinophils were subsequently cultured for 120 min. Results are presented as the proportion of IL-5Rα positive eosinophils.