Table 2.
Cytokine profile of the supressor T cell lines
| Line TS1 | Line TS2 | |||
|---|---|---|---|---|
| ConA* | T cell clones† | ConA* | T cell clones† | |
| IL-2‡ | <0·02 | <0·02 | <0·02 | <0·02 |
| IL-4 | <0·02 | <0·02 | <0·02 | <0·02 |
| IL-5 | <0·1 | <0·1 | <0·1 | <0·1 |
| IL-10 | ND | 3·87 | ND | 2·58 |
| TGF-β | 2·4 | 4·2 | 4·1 | 1·1 |
| IFN-γ | ND | 3 | ND | 0·42 |
2·5 × 106 cells from either lineage were stimulated with syngeneic APC and 2·5 µg/ml of ConA. Supernatants were harvested 24 and 48 h later and their cytokines were evaluated as described in Materials and methods. The higher level of each measure is presented.
A mixture of equal proportions of T cell KLH-specific irradiated clones (2·5 × 106 cells) was irradiated (3300 rads) and added to the same amount of cells from either lineage in 24 well plate. Two days prior to the irradiation, T cell clones were stimulated with the pertinent antigen plus irradiated (2500 rads) syngeneic APC. Supernatants were collected and assayed for the shown cytokines as described.
Maximal cytokine measured in cell culture media, presented in ng/ml. ND, Not done.