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IL-8, MCP-1 and RANTES mRNA expression in human renal epithelial cells, A498 and primary HMC by RT-PCR after stimulation with E. coli CFT-073 for 0, 2, 6 and 24 h. A chemokine/G3PDH band intensity ratio was calculated for each chemokine at each time point (upper panel). Bands are displayed at the different time points for each chemokine (lower panel).
