Table 3.

Analysis of the complement pathways involved in lysis of CA cells by antibody and human serum

	Inhibition of lysis (%)†
Cells*	RB	MgEGTA-NHS	Anti-MBL (3F8)-NHS
T47D	49	30	12
SKOV3	38	38	15
PC-3	45	34	18
NA-T47D	65	39	‡ns
NA-SKOV3	68	37	‡ns
NA-PC-3	69	35	ns

^*Cells were treated first with neuraminidase (NA) or PBS (control) and then lysed by antibody and serum. Percentage lysis was determined by trypan blue inclusion.

^†Percentages presented indicate a significant (P < 0·05) inhibition of cell lysis relative to control NHS. NHS, normal human serum; RB, human serum depleted of factor B; MgEGTA, 2·5 mm MgCl₂ and 10 mm EGTA in NHS; 3F8, 67 µg/ml 3F8 mAb in NHS.

^‡No significant inhibition.