Fig. 1.

Flow cytometry analysis. (a) Monocytes: monocytes were gated on size and granularity and analysed for cytokine production by double labelling with anti-CD14-FITC and either anti-TNF-α-PE or anti IL-12-PE antibodies. (b) Th lymphocytes: lymphocytes were gated on size and granularity and CD4⁺ lymphocyte production of IFN-γ and IL-4 was analysed by triple labelling with anti-CD4-APC, anti-IFN-γ-FITC and anti IL4-PE. Single colour plots were gated for CD4⁺ lymphocytes and two colour plots of these cells labelled for IL-4 and IFN-γ are shown. (c) NK cells: lymphocytes were gated on size and granularity and NK cell production of IFN-γ was analysed by double-labelling with anti CD56-PE and anti IFN-γ-FITC.