Fig. 3.

CD4⁺ T-helper cell production of intracellular IFN-γ and IL-4 PBMCs from six healthy non-pregnant and six normal pregnant women were incubated for 6 h with monensin (2·3 µm) alone, or with the additional presence of stimulants PMA (40 ng/ml) and calcium ionophore A23187 (1 µm). Cells were triple-labelled for CD4 and intracellular IFN-γ and IL-4, and 60 000 events analysed by flow cytometry. The percentage of CD4⁺ cells in the lymphocyte gate is shown for non-pregnant and pregnant women (a) The percentage of CD14⁺ cells which were IFN-γ positive (b) and IL-4 positive (c) was calculated. Significant differences are shown between non-pregnant and pregnant groups (*P < 0·05). The ratio of IFN-γ : IL-4 producing cells (d) was not significantly different between the two groups. □, Non-pregnant control (n = 6); ▪, normal pregnant subjects (n = 6).