Fig. 4.

Natural killer (NK) cell production of intracellular IFN-γ PBMCs from healthy six non-pregnant and six normal pregnant women were incubated for 6 h with monensin (2·3 µm) alone, or with the additional presence of stimulants PMA (40 ng/ml) and calcium ionophore A23187 (1 µm). Cells were double-labelled for CD56 and intracellular IFN-γ, and 60 000 events analysed by flow cytometry. Significant differences between non-pregnant and pregnant groups in the percentages of IFN-γ producing cells in the lymphocyte gate are shown (**P < 0·01). □, Non-pregnant control (n = 6); ▪, normal pregnant subjects (n = 6).