Table 1.

Antibodies for labelling leucocytes

	Antibody
Antigen	Location	Subtype	Dilution	Conjugate label
Monocytes
CD14	Surface	IgG2b	1 : 4	FITC*
TNF-α	Intracellular	IgG1	1 : 4	PE†
IL-12	Intracellular	IgG1	1 : 8	PE†
Lymphocytes
CD4	Surface	IgG1	Neat	Unconjugated‡
CD56	Surface	IgG1	Neat	PE‡
IFN-γ	Intracellular	IgG1	1 : 40	FITC†
IL-4	Intracellular	IgG1	1 : 4	PE†

^*Coulter, Luton, UK (My4 epitope).

^†Pharmingen, Cambridge Bioscience, Cambridge, UK

^‡Serotec, Kidlington, UK