Fig. 2.

Jak-1 phosphorylation is inhibited by anti-CD4 MoAb and HIV env in CD4 T cells. Cells were treated as indicated in the legend to Fig. 1. Jak-1 activation status in cells treated with (+) or without (–) IL-2 was evaluated by immunoprecipitation (IP) with anti-Jak-1 antibody and Western blotting (WB) using anti-P-Tyr MoAb (4G10) and anti-Jak-1 antibody as described in Materials and methods. Densitometry results are shown as in Fig. 1.