Fig 3.

In vitro dose–response of the expression of various genes in peripheral blood eosinophils of healthy volunteer after cytokine stimulation. The expression of CD44 (a), GM-CSF receptor α chain (b), GM-CSF receptor β chain (c), IL-3 receptor α chain (d), PAF receptor (e), bcl-2 (f), bcl-xL (g) and IL-5 receptor α chain (h)after a 3-h stimulation with the indicated concentrations of IL-5 (red bar), IL-4 (blue bar), IFN-γ (yellow bar), GM-CSF (green bar) and eotaxin (brown bar) is shown. An arbitrary unit contrasted with the unstimulated control level (standardized to levels of GAPDH transcript) is shown on the ordinate. These experiments were performed three times. The mean ± standard error of data from three independent experiments for each stimulation condition and the statistical results of the paired t-test are shown (*P < 0·05, **P < 0·01, ***P < 0·001).