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Comparison of HLA-A2/pp65 tetramer staining with CMVpp65 peptide- and CMV lysate-pulsing techniques for the detection of CMV-specific T lymphocytes in HLA-A2-positive, CMV-seropositive healthy individuals. The frequency of CMV-specific T lymphocytes was highest using the lysate pulsing technique, followed by tetramer staining and CMVpp65 peptide pulsing. Four individuals did not display CMV-specific T cells using any of the techniques.
