Table 1.
Summary of results of various parameters of mice injected with rabbit anti mouse C1q or control IgG
| Experimental | Control | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | Average | 1 | 2 | 3 | 4 | 5 | Average | p value | |
| 24 h | |||||||||||||
| Rb IgG deposition | + + | + + | + + | + + | + + | + + | – | – | – | – | – | – | 0·03* |
| Ms C1q deposition | + + | + + | + + | + + | + + | + + | – | – | – | – | – | – | 0·03* |
| Ms C3 deposition | + + | + + | + + | + + | + + | + + | – | – | – | – | – | – | 0·03* |
| Ms IgG deposition | – | – | – | – | – | – | – | – | – | – | – | – | – |
| CD 45 pos cells | 0·7 | 0·2 | 2 | 0·5 | 0·85 | 0·85 | 0·7 | 0·6 | 0·7 | 0·6 | 0·6 | 0·64 | 0·748 |
| Glom. proliferation | – | – | – | – | – | – | – | + | – | – | – | – | 0·317 |
| Hyaline deposits | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Glom. sclerosis | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Albuminuria | 30 | 26·1 | 29·8 | 19·1 | 42·1 | 29·42 | 34·6 | 16·2 | 40·9 | 18·4 | 5·3 | 23·08 | 0·347 |
| Mouse no. | 6 | 7 | 8 | 9 | 10 | Average | 6 | 7 | 8 | 9 | 10 | Average | P-value |
| 2 weeks | |||||||||||||
| Rb IgG deposition | + + + | + + + | + + + | + + + | + + + | + + + | + | + | + | + | + | + | 0·03* |
| Ms C1q deposition | + + + | + + + | + + + | + + + | + + + | + + + | + | + | + | + | + | + | 0·03* |
| Ms C3 deposition | + | + | + | + | + | + | – | – | – | – | – | – | 0·03* |
| Ms IgG deposition | + + | + + | + + | + + | + + | + + | + | + | + | + | + | + | 0·03* |
| CD 45 pos cells | 3·7 | 1·9 | 2·2 | 2·1 | 2·5 | 2·48 | 1·9 | 2·2 | 1·2 | 0·7 | 1·3 | 1·46 | 0·046* |
| Glom. proliferation | + | + | + | + | + | + | + | + | + | + | + | + | – |
| Hyaline deposits | – | + | + | – | – | – | – | – | – | – | – | – | 0·134 |
| Glom. sclerosis | – | – | + | – | – | – | – | – | – | – | – | – | 0·317 |
| Albuminuria | 4·8 | 12·6 | 364·8 | 30·7 | 86·1 | 99·8 | 59·8 | 7·9 | 15·3 | 32·8 | 7·5 | 24·66 | 0·602 |
Immunofluorescence intensities were scored as – (absent), + (positive), + + (strongly positive), + + + (brightly positive) (for statistical analysis scored as 0, 1, 2, 3).CD45-positive cells, as a marker for leucocytes, were counted in at least 10 glomeruli and expressed as absolute numbers of CD45-positive cells per glomerulus.Glomerular cell proliferation, hyaline deposits and glomerular sclerosis were scored as (–), absent,(+) present in less than 25% of the glomeruli,(+ +), 25–50% or (+ + +), more than 50% of the glomeruli (for statistical analysis scored as 0, 1, 2, 3). Renal injury was quantified by measuring 24 h urinary albumin excretion, data expressed as ug albumin per 24 h. Statistical analysis was performed by testing mice injected with rabbit antimouse C1q against those that received rabbit IgG in a Mann–Whitney test.
Significant difference between groups.