Fig. 3.

Specificity of binding of sera to recombinant hdsg3 and mdsg3. Increasing concentrations of recombinant hdsg3 or mdsg3 were mixed with 1 : 200 diluted of sera from mice immunized with hdsg3 or mdsg3 protein and incubated for 1 h at room temperature. The mixture was then assayed using an ELISA. (a) Reactivities of anti-mdsg3 and anti-hdsg3 antibodies against hdsg3 after preincubating with increasing concentrations of hdsg3. (b) Reactivities of anti-mdsd3 and anti-hdsg3 antibodies against mdsg3 after preincubating with increasing concentrations of mdsg3. □, Mouse mdsg3 sera; ⋄, mouse hdsg3 sera.